Prabir pondered this. ‘The pigeon genome has probably been copied in the presence of SPP dozens of times. So whatever transformation SPP causes must be convergent: the genome must change less and less with each iteration, until by now it’s virtually stable under the process.’
Grant nodded. ‘Whereas there’s no reason at all why the test sequences they first tried copying would have been stable. Randomly chosen input sequences would have undergone apparently random changes.’
Prabir had a minor epiphany. ‘And all the different fruit pigeons on Banda that ended up looking identical—the process must also be convergent for sufficiently similar genomes. Not only is there a stable endpoint for a given starting point, but similar starting points—closely related species—get dragged towards the same endpoint.’ He beamed with delight. ‘It all makes sense!’
Grant was pleased, but slightly less rapturous. ‘Except we still don’t know what SPP actually does, or how it’s doing it.’
‘But the Brazilians have all the information they need to crack this now, don’t they? They just have to look more closely at their model.’
‘Maybe. For a molecule as large as a protein you can never solve the equations for its shape and binding properties exactly, and it can be hard to choose a set of approximations that only cause trivial discrepancies. They’ve already tried simulating the pigeon chromosome being copied by SPP, and the simulation produced exactly the same error rate as for any other sequence.’
Prabir winced. ‘So their model has just proved that it’s missing the most important subtlety of the real protein.’
Grant didn’t see it quite so bleakly. ‘Missing it now, but they might yet be able to capture it with a little fine-tuning. At least they know what they’re aiming for, what they need to get right.’
Prabir said, ‘OK. So what do we do next?’ Grant had been posting all their results on the net, stating precisely where they’d been collecting their samples; the expedition biologists would already know that there was no need for anyone else to come here. So long as Grant didn’t cut corners.
She said, ‘I’ll have a proper look at these pupae, see what that tells us. I don’t know whether it’s worth going back to hunt for the larvae; I mean, the life cycle is of interest in itself, but larvae don’t make germ cells.’
Prabir filled a bucket with sea water and set about washing his sap-stained clothes, while Grant went for a swim. The travel shop in Toronto had sold him a detergent with enzymes that worked in the presence of salt; as long as you didn’t leave it too late you could remove almost anything with the stuff.
When he walked back into the cabin to get fresh water for rinsing, he glanced at the wire cage holding the adult butterflies they’d captured.
There was a pupa, similar to the ones he’d collected in the forest, hanging from the top of the cage. Except it couldn’t be a pupa. The adults had only been there for a day; at most they might have laid eggs. Grant had been in the cabin twenty minutes before. This had happened since then.
Prabir counted the adults. One of them was missing.
He ran out on deck. ‘Martha! You have to see this!’
She was halfway to the reef. ‘See what?’
‘The butterflies.’
‘What about them?’
‘You won’t believe me if I tell you. You have to see it for yourself.’
Grant turned back towards the boat. She followed him into the cabin, dripping. Prabir watched her expression go through several changes.
He said, ‘There’s something I’ve been wanting to try, if you’ll let me.’
‘I’m listening.’
He picked up one of the dormant adults he’d taken from the forest. ‘This insect hangs there, looking like a nutmeg fruit, unable to fly away. So presumably it has some defence: it must smell bad, or taste bad, to the birds that would otherwise want to eat it on sight.’
‘Presumably.’
Prabir approached the cage where they’d placed the fruit pigeons, and gave Grant a questioning look.
She said, ‘Go ahead, please. I want to see this too.’
He opened the door just wide enough to toss the dormant adult on to the floor of the cage. All of the fruit pigeons rushed forward; one of them managed to shoulder the rest aside and grab the insect. The bird stretched its jaws to their full extent and swallowed the sleeping butterfly whole.
Grant sat down heavily on one of the stools. After a long silence, she declared, ‘Maybe there’s a parasitic larval stage. Maybe the adults don’t lay their fertilised eggs; maybe they’re incubated inside the pigeons, after the adults act as a lure.’
‘And that’s why we’ve seen no larvae?’
‘Maybe.’ Grant stretched her arms and leant back on the stool. ‘I suppose it could burrow out through the skin, but I’m beginning to have visions of sifting through a large pile of pigeon shit.’
Prabir walked over to the butterfly cage. They’d placed some foliage in the bottom, but there’d been no elevated twigs or branches from which the would-be martyr could hang itself. He squatted down to try to get a better view of it, and saw a long string of dark-grey beads sticking to the underside of one of the leaves.
He said, ‘Was this foliage clean when you put it in the cage?’
‘I believe so. Why?’
‘I think I’ve just found some butterfly eggs.’
Prabir lay awake, listening to the waves breaking on the reef. The eggs would allow them to observe every stage of the butterfly, but that still wouldn’t be enough. The butterfly’s genome would be stable now; only samples from the kampung could show the way the São Paulo protein had changed it, from generation to generation, twenty years before. They needed to extract every clue the island held; if they didn’t finish the job properly, the expedition would follow them here.
He went into the cabin and woke Grant, calling out to her from the doorway. Her bunk was hidden in shadows, but he heard her sit up. ‘What is it?’
He explained what he’d seen from the treetops. ‘I know where it is now. I can get to it from the beach.’
She hesitated. ‘Are you sure you want to do this? You could draw me a map, I could go by myself.’
Prabir was tempted. The place meant nothing to her: she could walk in and take whatever she needed, ransacking the site unflinchingly, immune to its history.
But this was his job. He couldn’t claim to be sparing Madhusree the pain of returning, only to hand the task over to a stranger.
‘I’d rather go alone.’
Grant said decisively, ‘We’ll go together, first thing tomorrow. I promised you after the mangrove swamp: we won’t get separated again.’
12
Prabir took comfort in the usual routine: wading to the beach, insect repellent, mine detector checks. Looking back at the reef as he pulled on his boots. They’d gather some samples and return to the boat. It would be a day like any other.
He’d estimated GPS coordinates for the kampung, from his notepad’s log of its position the previous day and his recollection of the treetop view. They picked their way laboriously through the shrubs; this was the first time they’d had no choice about their destination, no option of taking an easier route. Grant had once tried clearing a path in the undergrowth using a parang she’d bought in Ambon, but it had been a waste of effort; the machete was perfect for chopping through occasional vines, but the knee-high thicket was too tangled, there were too many strands to sever.
Grant was unusually quiet; she might have done this easily enough alone, but his presence must be making her feel more like a trespasser. Prabir said, ‘You wouldn’t believe it only took me half an hour a day to maintain this path.’
‘That was one of your jobs?’
‘Yeah.’
She smiled. ‘I thought I was hard done by having to clean the bath. And at least I had somewhere to spend my pocket money. I suppose you got paid in net privileges?’